Treatment of Domestic Wastewater by Enhanced Primary Decantation and Subsequent Naturally Ventilated Trickling Filtration

To treat household wastewater, a sequence of ‘primary decantation–trickling filter percolation’ was applied in a lab-scale designed treatment system. Poly-electrolyte was used as coagulant to enhance the primary treatment and charcoal was used as carrier material in the trickling filters. Oxygen was supplied to the trickling filters by means of natural ventilation. In the lab-scale system, the enhanced primary stage removed more than 91% of the suspended solids (SS), and 79% of the total chemical oxygen demand (CODt). The subsequent trickling filtration brought a complete nitrification to the wastewaters at a volumetric loading rate (Bv) of 0.7–1.0 g CODt L^-1 d^-1. On average, the concentrations of the CODt and SS in the final effluents were about 55 and 15 mg L^-1 respectively. With respect to phosphate, physico-chemical removal was the dominant process. About 46–62% of total P was removed from the tested wastewaters. The integrated treatment system also achieved a fair degree of hygienisation. The numbers of total coliforms, fecal coliforms and fecal streptococci were decreased by 2–4 log units. The sludge production of the entire treatment system was about 1.7% (v/v) of the treated wastewater. Only primary sludge was produced; secondary sludge produced in the trickling filters was negligible. The cost savings in terms of minimization of sludge production and aeration energy are estimated to be substantial (i.e. some 50%) relative to a conventional activated sludge system.     

Genetic population structure of marine fish: mismatch between biological and fisheries management units

An essential prerequisite of a sustainable fisheries management is the matching of biologically relevant processes and management action. In fisheries management and assessment, fish stocks are the fundamental biological unit, but the reasoning for the operational management unit is often indistinct and mismatches between the biology and the management action frequently occur. Despite the plethora of population genetic data on marine fishes, to date little or no use is made of the information, despite the fact that the detection of genetic differentiation may indicate reproductively distinct populations. Here, we discuss key aspects of genetic population differentiation in the context of their importance for fisheries management. Furthermore, we evaluate the population structure of all 32 managed marine fish species in the north‐east Atlantic and relate this structure to current management units and practice. Although a large number of studies on genetic population structure have been published in the last decades, data are still rare for most exploited species. The mismatch between genetic population structure and the current management units found for six species (Gadus morhua, Melanogrammus aeglefinus, Merlangius merlangus, Micromesistius poutassou, Merluccius merluccius and Clupea harengus), emphasizes the need for a revision of these units and questions the appropriateness of current management measures. The implementation of complex and dynamic population structures into novel and less static management procedures should be a primary task for future fisheries management approaches.     

In Vitro Polymerization of Wheat Glutenin Subunits with Inorganic Oxidizing Agents. I. Comparison of Single‐Step and Stepwise Oxidations of High Molecular Weight Glutenin Subunits

High molecular weight glutenin subunits (HMW‐GS) were isolated from wheat flour and polymerized in vitro at pH 3.0 with different oxidizing agents (KBrO₃, KIO₃, H₂O₂). An oxidation protocol with single addition of oxidant (single‐step oxidation) was compared with a set‐up in which the oxidant was added in multiple steps (stepwise oxidation). Changes in size distribution were evaluated with size‐exclusion HPLC, multilayer SDS‐PAGE, and flow‐field flow fractionation (flow‐FFF). Flow‐FFF is particularly suitable for measuring changes in glutenin size in the very high size ranges. In order of increasing sizes of the resulting polymers, the different oxidizing agents could be ranked as KBrO₃ < KIO₃ < H₂O₂. However, none of the oxidation conditions allowed for a complete polymerization of HMW‐GS. Interestingly, it was found that high concentrations of KIO₃ negatively affect the degree of polymerization. A similar observation was not made with KBrO₃ or H₂O₂. SDS‐PAGE showed that y‐type HMW‐GS particularly failed to incorporate in glutenin polymers. Simultaneously, these HMW‐GS displayed higher mobilities on SDS‐PAGE that can be ascribed to the formation of intrachain SS bonds. Possible explanations for the incomplete polymerization of HMW‐GS are given.